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This supports the contention that these pretreatments have an impact on the protein composition and substrate surroundings, As a result lessening hydrolysis more than when just one pretreatment is utilised.

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The diploma of hydrolysis on the samples was determined determined by the L-serine equivalent technique. The data ended up acquired from a few unbiased hydrolyses for each roe and protease combination. Assessment of variance (ANOVA) was completed. Letters a–c show substantial dissimilarities amongst diverse samples prepared with different enzyme concentrations simultaneously level (p < 0.05).

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